Phenylethanolamine A ELISA Test Kit

Principle

The test kit is based on the competitive enzyme immunoassay for the detection of Phenylethanolamine A in the sample. The conjugated antigen is pre-coated on the micro-well stripes. The Phenylethanolamine A in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti- Phenylethanolamine A antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Phenylethanolamine A concentration in the sample. The value is compared to the standard curve and the Phenylethanolamine A concentration is subsequently obtained.